Morphometric analysis was conducted on randomly selected glomeruli captured on TEM digital images. Transmission electron microscopy (TEM) was performed using kidney samples from rats with nephrotoxic serum nephritis (NSN) from day 1 to day 14. This study investigated the time course of glomerular ultrastructure in experimental CGN.
However, the sequential changes in glomerular ultrastructure in CGN are not well defined. In crescentic glomerulonephritis (CGN), the development of cellular bridges between podocytes and parietal epithelial cells (PECs) triggers glomerular crescent formation. The interaction between nephrin and Neph1 therefore appears to be an important determinant of glomerular permeability. The interaction between nephrin and Neph1 is specific and not shared by either protein with P-cadherin, another integral slit diaphragm protein. This disruption modestly reduces Neph1 and nephrin protein expression in podocytes and dramatically reduces ZO-1 protein expression via the interaction of ZO-1 PDZ domains with the cytoplasmic tail of Neph1, independent of changes in mRNA expression of all three genes. Disruption of the Neph1-nephrin interaction in vivo by injecting combinations of individual subnephritogenic doses of anti-Neph1 and anti-nephrin results in complement- and leukocyte-independent proteinuria with preserved foot processes. Both native and recombinant Neph1 associate with each other as dimers and multimers and interact with nephrin via their extracellular segments. In this paper, we localize the expression of Neph1 to the glomerular slit diaphragm by immunogold electron microscopy in rodents and describe its direct interaction with two other components of the slit diaphragm, nephrin and ZO-1. While the precise subcellular localization of Neph1 remains unknown, its relationship with other components of the glomerular filtration barrier is of great interest in this field.
mnt-by: RIPE-NCC-HM-MNT created: last-modified: source: RIPE % Information related to 'AS34788' % Abuse contact for 'AS34788' is aut-num: AS34788 as-name: NMM-AS descr: D - 02742 Friedersdorf Hauptstrasse 68 descr: Germany import: from AS174 accept ANY import: from AS1299 accept ANY import: from AS8928 accept ANY import: from AS3320 accept AS3320 import: from AS6695 accept ANY import: from AS3356 accept ANY import: from AS8422 accept AS8422 import: from AS13184 accept AS13184 import: from AS8365 accept AS8365 import: from AS-FREENETDE accept AS-FREENETDE import: from AS-NETUSE accept AS-NETUSE export: to AS174 announce AS-NMM export: to AS1299 announce AS-NMM export: to AS3320 announce AS-NMM export: to AS8928 announce AS-NMM export: to AS6695 announce AS-NMM export: to AS3356 announce AS-NMM export: to AS8422 announce AS-NMM export: to AS13184 announce AS-NMM export: to AS8365 announce AS-NMM export: to AS-FREENETDE announce AS-NMM export: to AS-NETUSE announce AS-NMM org: ORG-NMMG1-RIPE admin-c: RM1862-RIPE tech-c: RM1862-RIPE status: ASSIGNED mnt-by: RIPE-NCC-END-MNT mnt-routes: nmm-mnt mnt-by: nmm-mnt created: last-modified: source: RIPE organisation: ORG-NMMG1-RIPE org-name: Neue Medien Muennich GmbH org-type: LIR address: HAUPTSTRASSE 68 address: 02742 address: FRIEDERSDORF address: GERMANY phone: +493587235310 fax-no: +493587235330 admin-c: RM1862-RIPE mnt-ref: RIPE-NCC-HM-MNT mnt-ref: nmm-mnt mnt-by: RIPE-NCC-HM-MNT abuse-c: NMM33-RIPE created: last-modified: source: RIPE # Filtered person: Rene Muennich address: Neue Medien Muennich GmbH address: Hauptstrasse 68 address: D-02742 Friedersdorf phone: +49 35872 353 10 fax-no: +49 35872 353 30 nic-hdl: RM1862-RIPE mnt-by: nmm-mnt created: last-modified: source: RIPE # Filtered % This query was served by the RIPE Database Query Service version 1.91.Neph1-deficient mice develop nephrotic syndrome at birth, indicating the importance of this protein in the development of a normal glomerular filtration barrier. % Information related to 'AS33792 - AS35839' as-block: AS33792 - AS35839 descr: RIPE NCC ASN block remarks: These AS Numbers are assigned to network operators in the RIPE NCC service region. % To receive output for a database update, use the "-B" flag. % See % Note: this output has been filtered. % % The RIPE Database is subject to Terms and Conditions. % This is the RIPE Database query service.
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